Anti -Histone H2A.X Rabbit pAb

價格：在線咨詢

貨號：

KT012183

產(chǎn)品規(guī)格：

Applications：

WB/IHC/IF

Species：

H/M/R,H/M/R,H/M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	Histone H2A.X rabbit polyclonal
Protein full name	Histone H2AX
Synonyms	H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x
Immunogen	KLH conjugated Synthetic peptide corresponding to Human Histone H2A.X
Isotype	IgG
Purity	Affinity purification
Predicted MW.	15 kDa
Observed MW.	15 kDa
Subcellular location	Nucleus
Uniprot ID	P16104, P27661

Applications

applications	species	dilution	Positive tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	liver, kidney, colon
IHC	Human, Mouse, Rat	1: 1000-1: 2000	colon cancer, colon, kidney, testis
IF	Human, Mouse, Rat	1: 500-1: 1000	colon cancer, colon, kidney, testis

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Images

	Western blot analysis of H2AX (GB111508) at dilution of 1: 1000 Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse colon tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat kidney tissue lysate
	Immunohistochemistry analysis of paraffin-embedded human colon cancer using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse colon using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat colon using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111508) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat testis using H2AX (GB111508) at dilution of 1: 2000
	Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using H2AX (GB111508) at dilution of 1: 600
	Immunofluorescent analysis of paraformaldehyde-fixed rat colon using H2AX (GB111508) at dilution of 1: 600
	Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111508) at dilution of 1: 600
	Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111508) at dilution of 1: 600

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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