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Anti -ZAP70 Rabbit pAb

價(jià)格: 在線咨詢
貨號:
DRF101233
產(chǎn)品規(guī)格:
100 μL
Applications:
IHC/IF
Species:
M/R,M/R
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

ZAP70 rabbit polyclonal

Protein full name

Tyrosine-protein kinase ZAP-70

Synonyms

70 kDa zeta-chain associated protein, Srk, Syk-related tyrosine kinase, Zap70, Zap-70

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse ZAP70

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Cytoplasm

Uniprot ID

A0A0R4J8U1




Applications


Applications

Species

Dilution

Positive Tissue

IHC

Mouse, Rat

1: 650-1: 1300

spleen

IF

Mouse, Rat

1: 1000-1: 2000

spleen, lymph, thymus




Background


This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.




Images


Immunohistochemistry analysis of paraffin-embedded rat spleen using ZAP70 (GB111109) at dilution of 1: 1300

Immunofluorescent analysis of paraffin-embedded rat spleen using ZAP70 (GB111109) at dilution of 1: 2000

Immunofluorescent analysis of paraffin-embedded rat lymph using ZAP70 (GB111109) at dilution of 1: 1300

Immunofluorescent analysis of paraffin-embedded rat thymus using ZAP70 (GB111109) at dilution of 1: 1300




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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